{"id":182,"date":"2014-10-30T15:27:55","date_gmt":"2014-10-30T15:27:55","guid":{"rendered":"http:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/?p=182"},"modified":"2014-10-30T15:27:55","modified_gmt":"2014-10-30T15:27:55","slug":"10-29-14","status":"publish","type":"post","link":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/2014\/10\/30\/10-29-14\/","title":{"rendered":"10.29.14"},"content":{"rendered":"<h1 id=\"article-title-1\">Wigwams: identifying gene modules co-regulated across multiple biological conditions<\/h1>\n<div class=\"contributors\">\n<ol id=\"contrib-group-1\" class=\"contributor-list\">\n<li id=\"contrib-1\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Krzysztof+Polanski&amp;sortspec=date&amp;submit=Submit\">Krzysztof Polanski<\/a><\/span><a id=\"xref-aff-1-1\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-fn-2-1\" class=\"xref-fn\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#fn-2\"><sup>\u2020<\/sup><\/a>,<\/li>\n<li id=\"contrib-2\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Johanna+Rhodes&amp;sortspec=date&amp;submit=Submit\">Johanna Rhodes<\/a><\/span><a id=\"xref-aff-1-2\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-fn-2-2\" class=\"xref-fn\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#fn-2\"><sup>\u2020<\/sup><\/a><a id=\"xref-fn-1-1\" class=\"xref-fn\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#fn-1\"><sup>\u2021<\/sup><\/a>,<\/li>\n<li id=\"contrib-3\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Claire+Hill&amp;sortspec=date&amp;submit=Submit\">Claire Hill<\/a><\/span><a id=\"xref-aff-1-3\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>2<\/sup><\/a>,<\/li>\n<li id=\"contrib-4\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Peijun+Zhang&amp;sortspec=date&amp;submit=Submit\">Peijun Zhang<\/a><\/span><a id=\"xref-aff-1-4\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>2<\/sup><\/a>,<\/li>\n<li id=\"contrib-5\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Dafyd+J.+Jenkins&amp;sortspec=date&amp;submit=Submit\">Dafyd J. Jenkins<\/a><\/span><a id=\"xref-aff-1-5\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a>,<\/li>\n<li id=\"contrib-6\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Steven+J.+Kiddle&amp;sortspec=date&amp;submit=Submit\">Steven J. Kiddle<\/a><\/span><a id=\"xref-aff-1-6\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-fn-3-1\" class=\"xref-fn\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#fn-3\"><sup>\u00a7<\/sup><\/a>,<\/li>\n<li id=\"contrib-7\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Aleksey+Jironkin&amp;sortspec=date&amp;submit=Submit\">Aleksey Jironkin<\/a><\/span><a id=\"xref-aff-1-7\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a>,<\/li>\n<li id=\"contrib-8\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Jim+Beynon&amp;sortspec=date&amp;submit=Submit\">Jim Beynon<\/a><\/span><a id=\"xref-aff-1-8\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-aff-1-9\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>2<\/sup><\/a>,<\/li>\n<li id=\"contrib-9\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Vicky+Buchanan-Wollaston&amp;sortspec=date&amp;submit=Submit\">Vicky Buchanan-Wollaston<\/a><\/span><a id=\"xref-aff-1-10\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-aff-1-11\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>2<\/sup><\/a>,<\/li>\n<li id=\"contrib-10\" class=\"contributor\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Sascha+Ott&amp;sortspec=date&amp;submit=Submit\">Sascha Ott<\/a><\/span><a id=\"xref-aff-1-12\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a> and<\/li>\n<li id=\"contrib-11\" class=\"last\"><span class=\"name\"><a class=\"name-search\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/search?author1=Katherine+J.+Denby&amp;sortspec=date&amp;submit=Submit\">Katherine J. Denby<\/a><\/span><a id=\"xref-aff-1-13\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>1<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-aff-1-14\" class=\"xref-aff\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#aff-1\"><sup>2<\/sup><\/a><span class=\"xref-sep\">,<\/span><a id=\"xref-corresp-1-1\" class=\"xref-corresp\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#corresp-1\">*<\/a><\/li>\n<\/ol>\n<p class=\"affiliation-list-reveal\"><a class=\"view-more\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#\">+<\/a> Author Affiliations<\/p>\n<ol class=\"affiliation-list hideaffil\">\n<li class=\"aff\"><a id=\"aff-1\" name=\"aff-1\"><\/a><br \/>\n<address><sup>1<\/sup>Warwick Systems Biology Centre and <sup>2<\/sup>School of Life Sciences, University of Warwick, CV4 7AL, UK<\/address>\n<\/li>\n<\/ol>\n<ol class=\"corresp-list\">\n<li id=\"corresp-1\" class=\"corresp\"><a class=\"rev-xref\" href=\"http:\/\/bioinformatics.oxfordjournals.org\/content\/30\/7\/962.full#xref-corresp-1-1\">\u21b5<\/a>*To whom correspondence should be addressed.<\/li>\n<\/ol>\n<ul class=\"history-list\">\n<li class=\"received\"><span class=\"received-label\">Received <\/span>September 17, 2013.<\/li>\n<li class=\"rev-recd\"><span class=\"rev-recd-label\">Revision received <\/span>December 12, 2013.<\/li>\n<li class=\"accepted\"><span class=\"accepted-label\">Accepted <\/span>December 13, 2013.<\/li>\n<\/ul>\n<\/div>\n<div id=\"abstract-1\" class=\"section abstract\">\n<h2>Abstract<\/h2>\n<p id=\"p-6\"><strong>Motivation:<\/strong> Identification of modules of co-regulated genes is a crucial first step towards dissecting the regulatory circuitry underlying biological processes. Co-regulated genes are likely to reveal themselves by showing tight co-expression, e.g. high correlation of expression profiles across multiple time series datasets. However, numbers of up- or downregulated genes are often large, making it difficult to discriminate between dependent co-expression resulting from co-regulation and independent co-expression. Furthermore, modules of co-regulated genes may only show tight co-expression across a subset of the time series, i.e. show condition-dependent regulation.<\/p>\n<p id=\"p-7\"><strong>Results:<\/strong> Wigwams is a simple and efficient method to identify gene modules showing evidence for co-regulation in multiple time series of gene expression data. Wigwams analyzes similarities of gene expression patterns within each time series (condition) and directly tests the dependence or independence of these across different conditions. The expression pattern of each gene in each subset of conditions is tested statistically as a potential signature of a condition-dependent regulatory mechanism regulating multiple genes. Wigwams does not require particular time points and can process datasets that are on different time scales. Differential expression relative to control conditions can be taken into account. The output is succinct and non-redundant, enabling gene network reconstruction to be focused on those gene modules and combinations of conditions that show evidence for shared regulatory mechanisms. Wigwams was run using six <em>Arabidopsis<\/em> time series expression datasets, producing a set of biologically significant modules spanning different combinations of conditions.<\/p>\n<p id=\"p-8\"><strong>Availability and implementation:<\/strong> A Matlab implementation of Wigwams, complete with graphical user interfaces and documentation, is available at: warwick.ac.uk\/wigwams.<\/p>\n<p id=\"p-9\"><strong>Contact:<\/strong> <a href=\"mailto:k.j.denby@warwick.ac.uk\">k.j.denby@warwick.ac.uk<\/a><\/p>\n<p id=\"p-10\"><strong>Supplementary Data:<\/strong> <a href=\"http:\/\/bioinformatics.oxfordjournals.org\/lookup\/suppl\/doi:10.1093\/bioinformatics\/btt728\/-\/DC1\">Supplementary data<\/a> are available at <em>Bioinformatics<\/em> online.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Wigwams: identifying gene modules co-regulated across multiple biological conditions Krzysztof Polanski1,\u2020, Johanna Rhodes1,\u2020\u2021, Claire Hill2, Peijun Zhang2, Dafyd J. Jenkins1, Steven J. Kiddle1,\u00a7, Aleksey Jironkin1, Jim Beynon1,2, Vicky Buchanan-Wollaston1,2, Sascha Ott1 and Katherine J. Denby1,2,* + Author Affiliations 1Warwick Systems Biology Centre and 2School of Life Sciences, University of Warwick, CV4 7AL, UK \u21b5*To whom [&hellip;]<\/p>\n","protected":false},"author":88,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3,13],"tags":[65,66,68,4,67,64],"_links":{"self":[{"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/posts\/182"}],"collection":[{"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/users\/88"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/comments?post=182"}],"version-history":[{"count":1,"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/posts\/182\/revisions"}],"predecessor-version":[{"id":183,"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/posts\/182\/revisions\/183"}],"wp:attachment":[{"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/media?parent=182"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/categories?post=182"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.discovery.wisc.edu\/sysbiojournalclub\/wp-json\/wp\/v2\/tags?post=182"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}